Changes in the model membrane structure induced by ribonuclease and lysozyme studied by the fluorescent probe technique
Gorbenko, G.P.
Biofizika 44(6): 1048-1053
1999
ISSN/ISBN: 0006-3029 PMID: 10707279 Document Number: 511495
Using fluorescent probes DSM and DSP-12, the effect of ribonuclease and lysozyme on the structural state of liposomes composed of phosphatidylcholine and diphosphatidylglycerol was studied. A correlation between the changes in probe quantum yield and the amount of protein-bound lipids was established. It is assumed that the formation of protein-lipid complexes increases the packing density of lipids and restricts their mobility. As the content of diphosphatidylglycerol in the lipid bilayer increases, the condensing effect of proteins becomes more pronounced.