Characteristics of the in vitro stimulation of horse leucocytes by phytohemagglutinin and antigen
Lazàry, S.; de Weck, A.L.; Gerber, H.; Schatzmann, U.; Straub, R.
Zeitschrift für Immunitatsforschung Experimentelle und Klinische Immunologie 145(4): 364-375
1973
ISSN/ISBN: 0300-872X PMID: 4282955 Document Number: 5073
The in vitro stimulation of horse leucocytes by phytohemagglutinin P and specific antigens was investigated. The stimulatory effect was expressed by the incorporation of 81I-thymidine into the cells. Cultures containing between 1.25 to 2.5 μg/ml PHA and 15 to 30% autologous serum showed the most effective stimulation. The response to PHA was maximal from the 3rd to the 7th day of culture. Cultures containing 0.125 to 2 × 10' cells per ml of medium gave proportionately similar responses to PHA. Circulating leucocytes from young foals, in contrast to cells from adult horses, responded very weakly to PHA. Leucocytes from a horse sensitized to diphtheria anatoxin manifested a dosedependent response to the antigen. The peripheral leucocytes of all animals tested responded to old tuberculin (bovine type).
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