Characteristics of the in vitro stimulation of horse leucocytes by phytohemagglutinin and antigen

Lazàry, S.; de Weck, A.L.; Gerber, H.; Schatzmann, U.; Straub, R.

Zeitschrift für Immunitatsforschung Experimentelle und Klinische Immunologie 145(4): 364-375

1973


ISSN/ISBN: 0300-872X
PMID: 4282955
Document Number: 5073
The in vitro stimulation of horse leucocytes by phytohemagglutinin P and specific antigens was investigated. The stimulatory effect was expressed by the incorporation of 81I-thymidine into the cells. Cultures containing between 1.25 to 2.5 μg/ml PHA and 15 to 30% autologous serum showed the most effective stimulation. The response to PHA was maximal from the 3rd to the 7th day of culture. Cultures containing 0.125 to 2 × 10' cells per ml of medium gave proportionately similar responses to PHA. Circulating leucocytes from young foals, in contrast to cells from adult horses, responded very weakly to PHA. Leucocytes from a horse sensitized to diphtheria anatoxin manifested a dosedependent response to the antigen. The peripheral leucocytes of all animals tested responded to old tuberculin (bovine type).

Document emailed within 1 workday
Secure & encrypted payments

Characteristics of the in vitro stimulation of horse leucocytes by phytohemagglutinin and antigen