Cloning and sequence analysis of 55 K protein of egg drop syndrome virus
Zhu, L.; Jin, Q.; Zeng, L.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 13(2): 145-147
1999
ISSN/ISBN: 1003-9279 PMID: 12569783 Document Number: 504983
For understanding the characteristics of genomic structure of egg drop syndrome virus(EDSV). Nucleic acid was extracted using routine method from weak virulent strain AA-2 of EDSV isolated from Chinese sick hens. Construction of the whole genomic library was by hydrolysis with Hind III, strand encoding 55 K gene locating in Hind III--A segment was sequenced and analyzed. The open reading frame has a length of 1,014 nt and codes a polypeptide of 337 amino acids with molecular weight of 38,200. Analysis of the amino acid sequence revealed a homology from 25.5%-32.4% to the 55 K protein of human adenovirus types 2, 12, 40, canine adenovirus and fowl adenoviruses of group 1, whereas to ovine adenovirus is 46.4%. The genomic structure of EDSV has some relationship with adenoviruses.