Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra
Hackbarth, C.J.; Unsal, I.; Chambers, H.F.
Antimicrobial Agents and ChemoTherapy 41(5): 1182-1185
1997
ISSN/ISBN: 0066-4804 PMID: 9145897 Document Number: 481954
A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.
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