Simultaneous determination of norepinephrine and epinephrime in plasma by using high performance liquid chromatograph
Zou, Y.; Qin, Y.; Liang, M.; Yu, Q.; Huang, Y.
Hua Xi Yi Ke da Xue Xue Bao 30(4): 457-459
1999
ISSN/ISBN: 0257-7712 PMID: 11387970 Document Number: 502564
A sensitive and rapid method was reported for the determination of nerepinephrine (NE) and epinephrine (E) in plasma by using high-performance liquid chromatography with an electrochemical detector (HPLC-EC) and YWG-C18 column(5u). 3,4-Dihydroxybenzylamine(DHBA) was used as an internal standard. The mobile phase of 0.05 mol/L actate buffer(pH3.0)-methanol-0.05 mol/L EDTA-Na2(78:20:2) with of 200 mg/L SDS solution was pumped through the column with a flow rate of 1.0 ml/min. The lengths of retention time for NE, E and DHBA(IS) was 7.3 min, 8.3 min and 10.4 min, respectively. The standard curve was linear within the concentration range of 31.25 to 4000 ng/L for NE and E (NE: r = 0.9999 E: r = 0.9996). The within day RSD (%) were less than 1.87% for NE and less than 2.07% for E, inter-day RSD(%) were less than 8.92% for NE and less than 9.65% for E respectively. The average extraction recovery(%) for NE and E were 93.5% +/- 5.50% and 91.3% +/- 1.77% respectively. This method has been applied to the diagnosis of pheochromocytoma and clinical research.