Research of the extraction method of morphine from biological fluids
Meng, P.J.; Wang, J.H.; Jiang, Z.L.; Yao, L.J.; Wang, Y.J.
Fa Yi Xue Za Zhi 15(1): 17-18; 63
1999
ISSN/ISBN: 1004-5619 PMID: 12536391 Document Number: 498973
A solid-phase extraction method of morphine from urine and blood has introduced. The effect of 5 SPE columns, 3 eluents and pH on morphine recovery has been investigated systematically. Derivative GC was used as a method of detection. The result showed that the column and the eluent of such as GDX-301, GDX-403 and C18 chloroform:isopropanol (9:1) had good behaviors to extraction of morphine. When GDX-301 was used as a sorbent, the recovery of morphine from urine was above 90% at pH 9, then went down with the increase of pH. While the recovery from blood was growing with the increase of pH, which reached above 90% in strong alkaline. The extraction method is simple, inexpensive, efficient and reproducible, which provides an effective and practical method to extract morphine and similar illicit drugs from biological fluids.