Microbiological assay of vitamin B12 in biological fluids. The Lactobacillus leichmannii method
Hansen, T.; Hauschildt, E.
Scandinavian Journal of Gastroenterology. Supplement 29: 27-31
1974
ISSN/ISBN: 0085-5928 PMID: 4530456 Document Number: 70427
Microbiological assay procedures for cobalamin are considered and the relative merits of the Euglena gracilis and Lactobacillus leichmannii methods are compared. A detailed description of the L. leichmannii method, as used in the Dumex laboratories, Copenhagen, is given. Serum cobalamin in 50 adults studied for 4 to 14 years ranged from 250 to 900 pg/ml. Accuracy and reproducibility of the method were both about plus or minus 10% for the normal range of serum cobalamin.