Top Abstract Purchase Related
Home
>
Node
>
Subnode

Thermostable alkaline metalloprotease from newly isolated alkalophilic Streptomyces diastaticus strain SS1

Chaphalkar, S.R.; Dey, S.

Indian Journal of Biochemistry and Biophysics 35(1): 34-40

1998

ISSN/ISBN: 0301-1208
PMID: 9699417
Document Number: 491283
Thermostable alkaline metalloprotease (EC 3.4.24.4), a simple protein of very low molecular mass (11 kDa), was isolated and purified from the cell free broth of Streptomyces diastaticus sp SS1 by concentration, (NH4)2SO4 precipitation and affinity chromatography over casein. The isoelectric point of the protease is 7.2. The temperature and pH optima for the enzyme activity are 37 degrees C and 8 respectively. The half life of the enzyme at 55 degrees C is 24 hr and the enzyme is stable over a pH range of 7.5 to 9. The K(m) value of the enzyme was estimated to be 2 x 10(-3) mg ml-1. Ca2+ is essential for enzyme activity as well as thermal stability. The alpha-helix content of the metalloprotease is calculated to be 50% from the circular dichroism spectrum. The number of Zn2+ binding to each molecule of protease is determined to be one by atomic absorption.

Document emailed within 1 workday
Secure & encrypted payments

Vijayalakshmi, B.; Subrahmanyam, A. 1993: A new alkalophilic variety of Streptomyces albus Hindustan Antibiotics Bulletin 35(3-4): 208-211
Bondarenko, B.M.; Lysenko, Z.A. 1970: Antibiotic substances isolated from Streptomyces endus strain P-6226 Mikrobiolohichnyi Zhurnal 32(4): 506-509
Orekhov, A.V.; Rebentish, B.A.; Lomovskaia, N.D. 1981: Restriction mapping of plasmid pSa1, isolated from Streptomyces albus G strain Molekuliarnaia Biologiia 15(6): 1258-1263
Tsai, Y.C.; Yamasaki, M.; Tamura, G. 1984: Substrate specificity of a new alkaline elastase from an alkalophilic bacillus Biochemistry International 8(2): 283-288
Dyková, I.; Fiala, I.; Pecková, H.; Dvoráková, H. 2008: Phylogeny of Flabellulidae (Amoebozoa: Leptomyxida) inferred from SSU rDNA sequences of the type strain of Flabellula citata Schaeffer, 1926 and newly isolated strains of marine amoebae Folia Parasitologica 55(4): 256-264
Tsurikova, N.V.; Nefedova, L.I.; Kostyleva, E.V.; Zvenigorodskiĭ, V.I.; Iasinovskiĭ, V.G.; Voeĭkova, T.A.; Sinitsyn, A.P. 2002: Preparation of an active strain of Bacillus licheniformis--producer of thermostable alpha-amylase Prikladnaia Biokhimiia i Mikrobiologiia 38(5): 502-508
Zhantieva, E.M.; Benediktova, A.B.; Feder, Z.M. 1978: Determination of the activity of thermostable alkaline phosphatase in the blood of pregnant women: a prognostic test of the status of the newborn infant Akusherstvo i Ginekologiia 9: 17-21
Liabakk, N.B.; Talbot, I.; Smith, R.A.; Wilkinson, K.; Balkwill, F. 1996: Matrix metalloprotease 2 (MMP-2) and matrix metalloprotease 9 (MMP-9) type IV collagenases in colorectal cancer Cancer Research 56(1): 190-196
Orlova, V.A.; Bogush, V.G.; Sharygin, A.A.; Danilenko, V.I. 1981: Identification and the physicochemical characteristics of the extrachromosomal DNA from a Streptomyces antibioticus strain Antibiotiki 26(9): 655-659
Ivanova, I.V.; Khristova, K.G.; Tishkov, S.K.; Moncheva, P.A. 1992: Effect of protoplasting on antibiotic activity and resistance in the strain Streptomyces hygroscopicus 155 Antibiotiki i Khimioterapiia 37(11): 3-5
Soldatova, L.I.; Sladkova, I.A.; Orekhov, A.V. 1994: Functioning of integrative vectors, based on phage phi C 31, in the strain Streptomyces bambergiensis 712 Antibiotiki i Khimioterapiia 39(6): 3-7
Davaadorzh, B.; Terekhova, L.P.; Tsétség, B.; Laĭko, A.V.; Puntsag, T. 1993: Streptomyces phaeofaciens, strain 51--producer of a novel antibiotic of the aureolic acid group Antibiotiki i Khimioterapiia 38(6): 11-14
Liutskaiuva, D.; Kamzolkina, O.V.; Starodubtseva, L.I.; Todorov, T.; Danilenko, V.N. 1991: Cytologic control of protoplast formation and regeneration in Streptomyces erythraeus, strain BTCC-2 Antibiotiki i Khimioterapiia 36(3): 8-11
Yang, H.; Li, M.; Jiang, Y. 1997: Molecular cloning and sequence analysis of glycoprotein D gene of herpes simplex virus 2 strain Sav and wild strain isolated Zhonghua Yi Xue Za Zhi 77(2): 97-99
Okunev, O.N.; Anan'in, V.M.; Golovlev, E.L.; Vinogradova, K.A.; Skriabin, G.K. 1978: Microbiological transformation of carbohydrates. Glucose isomerization into fructose by washed cells of Streptomyces sp. strain 29 Prikladnaia Biokhimiia i Mikrobiologiia 14(1): 44-49
Danilenko, V.N.; Akopiants, K.E.; Sizova, I.A.; Michurina, T.A. 1997: Determination of the nucleotide sequence and characterization of the novel aminoglycoside phosphotransferase aphVIIi gene from the Streptomyces rimosus strain Genetika 33(11): 1478-1486
el-Naggar, M.Y.; el-Kersh, M.A.; el-Sharaky, A.S. 1999: Correlation of actinomycin X2 to the lipid profile in static and shaken cultures of Streptomyces nasri strain YG62 Microbios 100(396): 117-127
Goto, T.; Kino, T.; Hatanaka, H.; Nishiyama, M.; Okuhara, M.; Kohsaka, M.; Aoki, H.; Imanaka, H. 1987: Discovery of FK-506, a novel immunosuppressant isolated from Streptomyces tsukubaensis Transplantation Proceedings 19(5 Suppl. 6): 4-8
Malanicheva, I.A.; Koz'mian, L.I.; Dudnik, I.V.; Florova, G.I.; Orekhov, A.V. 1992: Comparative study of Streptomyces--producer of aminoglycoside antibiotics, monomycin and kanamycin, and the strain 344 obtained by fusion of their protoplasts by the method of restrictive total DNA fingerprinting Antibiotiki i Khimioterapiia 37(5): 5-7
Imanishi, H.; Hada, T.; Muratani, K.; Hirano, K.; Higashino, K. 1990: Expression of a hybrid form of alkaline phosphatase isoenzyme in a newly established cell line (HuG-1) from a gastric cancer patient Cancer Research 50(11): 3408-3412