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Identification and the physicochemical characteristics of the extrachromosomal DNA from a Streptomyces antibioticus strain

Orlova, V.A.; Bogush, V.G.; Sharygin, A.A.; Danilenko, V.I.

Antibiotiki 26(9): 655-659

1981

ISSN/ISBN: 0003-5637
PMID: 7027917
Document Number: 168494
The data on centrifugation of DNA in the density gradient of caesium chloride-ethidium bromide, electron microscopy and electrophoresis of the DNA restricts from the satellite gradient band indicate that Str. antibioticus, the oleandomycin-producing organism possessed extrachromosomal cyclic DNA with the molecular weight of 21.3. 10(6) +/- +/- 0.3 10(6) daltons. The extrachromosomal cyclic DNA had 7 identification sites for endonuclease BamHI, 5 sites for PstI, 12 sites for PvuII, more than 14 sites for SmaI and non for endonucleases Eco RI and HindIII.

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