ELISA using enzyme penicillinase for the detection of IgG antibodies to Trypanosoma evansi in sera of experimentally infected rabbits
Bhopale, G.M.; Naik, S.R.
Hindustan Antibiotics Bulletin 40(1-4): 47-50
1998
ISSN/ISBN: 0018-1935 PMID: 16961206 Document Number: 487548
An Enzyme Linked Immunosorbent Assay (ELISA) using penicillinase as an enzyme marker has been developed for the detection of IgG antibodies in the sera of Trypanosoma evansi infected rabbits. Anti-T. evansi IgG antibodies were detected on 10th day after the infection and thereafter antibody titre rose progressively for 18 days. The developed assay is simple (end result assessed visually) and reproducible (4.4 and 14.0 percentage of intra and inter coefficient of variation respectively).
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