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Specific DNA probe for the sensitive detection of Trypanosoma evansi

Viseshakul, N.; Panyim, S.

Southeast Asian Journal of Tropical Medicine and Public Health 21(1): 21-27

1990

ISSN/ISBN: 0125-1562
PMID: 2402677
Document Number: 348152
DNA probes were constructed from T. evansi genomic DNA and kinetoplast DNA for sensitive detection of the parasite in infected blood. A 6.5 kb DNA insert of pMUTec6 plasmid derived from the genomic DNA of T. evansi Npl isolate, selected from 575 recombinant E. coli exhibited the strongest nucleic acid hybridization signal to the T. evansi DNA. Used as the DNA probe, pMUTec6 could detect as little as 60 pg T. evansi DNA and it did not hybridize to the DNA of cattle, water buffalo and 2 related blood parasites. A simple detection procedure by spotting 10 micro l of infected blood onto a nylon membrane could detect as little as 1000 parasites. The kinetoplast DNA was cloned in E. coli and found to show a comparable sensitivity to that of the pMUTec6. However, the kinetoplast DNA exhibited variation in copy number among parasite isolates.

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Bhopale, G.M.; Naik, S.R. 1998: ELISA using enzyme penicillinase for the detection of IgG antibodies to Trypanosoma evansi in sera of experimentally infected rabbits Hindustan Antibiotics Bulletin 40(1-4): 47-50
Mathieu-Daude, F.; Bicart-See, A.; Bosseno, M.F.; Breniere, S.F.; Tibayrenc, M. 1994: Identification of Trypanosoma brucei gambiense group I by a specific kinetoplast DNA probe American Journal of Tropical Medicine and Hygiene 50(1): 13-19
Gill, B.S.; Singh, J.; Gill, J.S.; Kwatra, M.S. 1987: Trypanosoma evansi infection in pigs in India Veterinary Record 120(4): 92
Mathur, S.C. 1972: Effect of 35 degrees C. on the course of Trypanosoma (Trypanozoon) evansi infection in mice Transactions of the Royal Society of Tropical Medicine and Hygiene 66(4): 526-527
de Oliveira, T.C.; Sogayar, R.; Salata, E. 1989: Serologic study of experimental infections by Trypanosoma evansi, in guinea pigs Revista do Instituto de Medicina Tropical de Sao Paulo 31(2): 95-99
Payne, R.C.; Sukanto, I.P.; Partoutomo, S.; Polytedi, F. 1992: Experimental infection of Friesian Holstein calves with an Indonesian isolate of Trypanosoma evansi Tropical Medicine and Parasitology: Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit 43(2): 115-117
Jones, T.W.; McKinnell, C.D. 1985: Antigenic variation in Trypanosoma evansi: a comparison of the predominant variable antigen type repertoire of stocks from Sudan Tropical Medicine and Parasitology: Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit 36(4): 205-209
Amoudi, M.A.; Al-Yousif, M.S.; Al-Shawa, Y. 2011: Morphological forms of Trypanosoma evansi from blood of Arabian camel (Camelus dromedarius) in the Riyadh metropolitan areas Journal of the Egyptian Society of Parasitology 41(1): 29-33
Kageruka, P.; Mortelmans, J. 1971: Isolation of Trypanosoma evansi Steel 1885 from a capybara (Hydrochoerus hydrochoeris Lin.) imported to Belgium and preliminary study of its virulence Annales des Societes Belges de Medecine Tropicale, de Parasitologie et de Mycologie 51(6): 709-716
Wessman, M.; Ruutu, T.; Volin, L.; Knuutila, S. 1989: In situ hybridization using a Y-specific probe--a sensitive method for distinguishing residual male recipient cells from female donor cells in bone marrow transplantation Bone Marrow Transplantation 4(3): 283-286
Gill, B.S. 1970: Comparative efficacy of techniques of recovering trypanosomes (Trypanosoma evansi) from the rat blood and methods of preparing antigen for use in the complement fixation test for surra Annales des Societes Belges de Medecine Tropicale, de Parasitologie et de Mycologie 50(5): 635-640
Geen, J.; Hullin, D.A.; Hogg, S.I. 1999: Detection of anti-Yta antibodies using a sensitive and specific enzyme-linked immunosorbent assay British Journal of Biomedical Science 56(2): 128-133
Kim, O.; Kim, S.S. 2003: A sensitive and specific detection of Human herpesvirus 8 by polymerase chain reaction and dot blot hybridization Acta Virologica 47(2): 87-90
Zhong, X.Y.; Lin, Y.S.; Kaul, S.; Bastert, G. 2000: Sensitive and Specific Detection of Carcinoembryonic Antigen cDNA Using the Hot Start Polymerase Chain Reaction Technique Clinical Laboratory 46(1-2): 7-11
Demel, U.; Tilz, G.P.; Foeldes-Papp, Z.; Gutierrez, B.; Albert, W.H.; Böcher, O. 2004: Detection of tumour cells in the peripheral blood of patients with breast cancer. Development of a new sensitive and specific immunomolecular assay Journal of Experimental and Clinical Cancer Research: Cr 23(3): 465-468
Bregni, M.; Siena, S.; Subar, M.; Villa, S.; Bonadonna, G.; Dalla-Favera, R.; Gianni, A.M. 1989: Detection of occult leukemic cells in the autologous bone marrow graft of a patient with T-cell acute lymphoblastic leukemia by a highly specific and sensitive assay Haematologica 74(1): 11-14
Grudzinskas, J.G.; Gordon, Y.B.; Jeffrey, D.; Chard, T. 1977: Specific and sensitive determination of pregnancy-specific beta1-glycoprotein by radioimmunoassay Obstetrical and Gynecological Survey 32(7): 578-579
Nakagawara, A.; Kayashima, K.; Tamada, R.; Onoue, K.; Ikeda, K.; Inokuchi, K. 1979: Sensitive and rapid method for determination of superoxide-generating activity of blood monocytes and its use as a probe for monocyte function in cancer patients Gan 70(6): 829-833
Cruz-Reyes, J.A.; Ackers, J.P. 1992: A DNA probe specific to pathogenic Entamoeba histolytica Archives of Medical Research 23(2): 271-275
Pelloux, I.; Croize, J.; Hirtz, P.; Comet, M.; Le Noc, P. 1991: Evaluation of a DNA probe test for the detection of Legionella SPP Pathologie-Biologie 39(2): 150-152