Immobilized cells with endocellular enzymes improved by the permealizing and crosslinking treatment
Shi, Y.; Wu, Y.; Jin, F.; Yan, F.; Yu, X.; Yuan, Q.
Chinese Journal of Biotechnology 13(1): 59-62
1997
ISSN/ISBN: 1042-749X PMID: 9376508 Document Number: 472363
In order to achieve high activity of enzymatic catalysis, the method of permealizing and crosslinking treatment has been suggested for immobilizing cells with endocellular enzymes. Polyethene-polyamine and glutardehyde were used to enhance the activity and stability of E. coli having aspartase entrapped in k-carrageenan gel and intact P. dacunhae cells having aspartate-beta-decarbonoxylase. The strength and enlargement effects, which favor the performance of catalysis in a packed bed reactor and a membrane reactor, respectively, were also obtained from the experiment results.