The effect of co-immobilized TNF-alpha/IFN-gamma on mitochondrial membrane potential of Hela cells
Zhong, L.; Wang, W.; Tao, H.; Guan, Y.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 26(5): 972-977
2009
ISSN/ISBN: 1001-5515 PMID: 19947470 Document Number: 631085
This study inquired into the mechanisms of co-immobilized cytokines and free cytokines-induced apoptosis on HeLa cells. With the use of photochemical fixed method, TNF-alpha/IFN-gamma were co-immobilized on a 24-well polystyrene culture plate. HeLa cells were stained with fluorescent probe JC-1 to detect the changes of mitochondrial membrane potential (deltapsim), and then were examined by flow cytometry. The results showed that co-immobilized cytokines could induce the apoptosis of HeLa cells in a dose-independent manner. When treated with low-dose of co-immobilized cytokines (20ng/ml), the mitochondrial membrane potential (deltapsim) of HeLa cells continually decreased in 6 days. These indicate that low dose co-immobilized cytokines have a long-term of apoptosis-inducing effect on HeLa cells. We assume that there is close relationship between the mitochondrial membrane potential decrease and the apoptosis of HeLa cells.