Cholesterol side-chain cleavage by immobilized cells of Rhodococcus equi DSM 89-133
Ahmad, S.; Roy, P.K.; Basu, S.K.; Johri, B.N.
Indian Journal of Experimental Biology 31(4): 319-322
1993
ISSN/ISBN: 0019-5189 PMID: 8359831 Document Number: 418316
Side-chain cleavage of sterol and extracellular cholesterol oxidase activity were investigated using viable cells of R. equi DSM 89-133 immobilized in polyacrylamide gel. In batch culture, immobilized cells were active in side-chain cleavage of cholesterol for more than 30 days. Free or immobilized cells were incapable of side-chain cleavage in the absence of 2,2' dipyridyl; cholesterol oxidase was, however, produced in both the cases. Maximal activity of the immobilized cells was 60 to 70% of the free cells.