Specific binding of human bone morphogenetic protein (2A) with mouse osteoblastic cells
Liu, X.; Chen, S.; Chen, N.; Gao, L.; Zhao, Z.
Chinese Medical Sciences Journal 11(2): 97-99
1996
ISSN/ISBN: 1001-9294 PMID: 9387417 Document Number: 467801
Human bone morphogenetic protein 2A (hBMP2A) cDNA terminal 567 nucleotides were cloned and expressed in a phage display vector pCSM21. Human BMP2A C-terminal peptide displayed on the surface of the phage can bind specifically to the surface of mouse osteoblastic cell (MC3T3) membrane. ELISA assay showed a positive signal of the binding by using antibody against M13 phage gene 8 protein. After labeling with 3HtdR, the counts of the binding groups were 3 to 10 times higher than the control groups. It suggests that the surface of MC3T3 cells exist the receptor for hBMP2A.