Content of bone morphogenetic protein-4 in human demineralized bone: relationship to donor age and ability to induce new bone formation
Honsawek, S.; Dhitiseith, D.
Journal of the Medical Association of Thailand 88(Suppl 4): S260-S265
2005
ISSN/ISBN: 0125-2208 PMID: 16623039 Document Number: 8607
Bone morphogenetic proteins (BMPs) are also called growth and differentiation factors (GDFs) and form a subfamily of related proteins within the TGF-beta superfamily. BMP-4 is one ofmultifuntional growth factors with pleiotropic roles in many different cell types and is predominantly present in human bone tissue. To analyze the content of extractable BMP-4 in human demineralized bone as a function of age. Bone samples were ground and demineralized by exposure to 0.5 N HCl and then extracted by collagenase digestion. Extractable BMP-4 was analyzed using a commercially available enzyme-linked immunosorbent assay (ELISA). 63 samples of demineralized bone matrix (DBM) derived from 36 men and 27 women between the ages of 15-65 years. The extractable BMP-4 content appears to be age-dependent, with DBM from younger donors being most likely to have higher BMP-4 quantity. In addition, DBM with high osteoinductivity contained greater amounts of extractable BMP-4 than DBM samples with low osteoinductivity. The BMP-4 in demineralized bone undergoes age-related decrease that may contribute to the reduction of bone volume observed with aging.
Document emailed within 1 workday
