A ciliary neurotrophic factor-sensitive human myeloma cell line
Gu, Z.J.; Zhang, X.G.; Hallet, M.M.; Lu, Z.Y.; Wijdenes, J.; Rossi, J.F.; Klein, B.
Experimental Hematology 24(10): 1195-1200
1996
ISSN/ISBN: 0301-472X PMID: 8765494 Document Number: 467122
We obtained a human myeloma cell line (XG4-CNTF) whose growth was completely dependent on addition of ciliary neurotrophic factor (CNTF). Half-maximal proliferation was induced by adding 20 pg/mL CNTF. Response to CNTF correlated with expression of membrane CNTF receptor alpha-chain (CNTFR apprx ), as shown by PCR analysis and immunostaining with anti-CNTFR-alpha antibodies. CNTF-induced proliferation was completely inhibited by antibodies to gp230 interleukin-6 (IL-6) transducer, unlike antibodies to IL-6 or IL-6 receptor (IL6R). Growth of XG4-CNTF cells using the gp130 IL-6 transducer was also supported by other cytokines: IL-6, leukemia inhibitory factor (LIF), and oncostatin M (OM). This cell line should be very useful for studying the interactions of IL-6-related cytokines with their receptors.