Establishment and characterization of a human squamous cell carcinoma cell line LK-52 which produce direct activator of coagulant factor X
Inufusa, H.; Hara, S.; Mori, N.; Yasutomi, M.
Human Cell 5(3): 287-291
1992
ISSN/ISBN: 0914-7470 PMID: 1467328 Document Number: 403068
Uterus origin squamous cell carcinoma cell line LK-52 was established from surgical specimen of lung metastatic nodule. LK-52 produce poorly differentiated squamous carcinoma in nude mouse, and doubling time in vitro was 38 hours. Chromosome analysis show various abnormality and main mode number was 67 and 68. LK-52 shed active procoagulant substance into culture medium. The culture medium of LK-52 shortening recalcification time of normal human plasma and factor VII or factor IX deficient plasma but not factor X deficient plasma. Procoagulant activity of LK-52 product may induced with direct activation of coagulant factor X. Procoagulant activity which produced by cancer cell may play a important roll in the unbalanced haemostasis of cancer patient.