Suppression of vascular endothelial growth factor expression by vector-based small interfering RNA in human tongue squamous carcinoma cell line Tca8113
Yu, D.-h.; Cao, Y.; Yao, Z.-w.; Li, J.; Chen, H.-b.; Hao, J.
Hua Xi Kou Qiang Yi Xue Za Zhi 26(5): 550-552
2008
ISSN/ISBN: 1000-1182 PMID: 19007083 Document Number: 618614
To assess suppression effects of vector-based small interfering RNA (siRNA) on vascular endothelial growth factor (VEGF) expression of human tongue squamous carcinoma cell line (Tca8113) in vitro. Two siRNA targeting VEGF constructed in eukaryotic expression vector (Pu-VEGF-siRNA1, Pu-VEGF-siRNA2), eukaryotic expression vector as the experiment control, all of which were transfected into Tca8113 cells with Lipofectamine 2000. Non-transfection cell was used as negative control. VEGF mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and enzyme linked immunosorbent assay (ELISA), respectively. Compared to the experimental and negative controls, the expression of VEGF mRNA and protein were significantly decreased in the Pu-VEGF-siRNA1 group and Pu-VEGF-siRNA2 group. But there were no significant differences between two controls (P > 0.05). Vector-based siRNAs targeting VEGF are efficient in down-regulating VEGF expression in Tca8113 cells.