Establishment and characterization of a human undifferentiated carcinoma cell line (HMG)
Sekiguchi, I.; Suzuki, M.; Tamada, T.; Murata, M.; Kawai, T.; Shinomiya, N.; Tsuru, S.; Kojima, M.
Human Cell 4(2): 150-156
1991
ISSN/ISBN: 0914-7470 PMID: 1720329 Document Number: 377803
The undifferentiated carcinoma cell line (HMG) was established from a nude mouse tumor which had been produced by transplantation of a intraperitoneal tumor of 27-year-old woman. The HMG cell line has the following biological properties. 1. The HMG cells are round to oval in shape and grow as floating cell aggregates like a rouleau or a cluster of grapes. 2. 100 passages have been carried out over a year, and the population doubling time is about 17 hrs. 3. In the original tumor, keratin and vimentin were expressed simultaneously, in HMG cells, however, only localization of vimentin was confirmed. 4. By chromosomal analysis, over 90% of the cells revealed 46, XX, with no karyological abnormalities, at passage 82. 5. When heterotransplanted into the subcutis of a nude mouse, HMG cells produced a undifferentiated carcinoma resembling the original tumor.