Establishment and characterization of the human uterine cervical epidermoid cancer cell line
Taguchi, S.
Nihon Sanka Fujinka Gakkai Zasshi 33(8): 1180-1188
1981
ISSN/ISBN: 0300-9165 PMID: 7196935 Document Number: 170785
A cell line designated SKG-1 was established in vitro from human uterine cervical epidermoid cancer which contained main components of clear cell epidermoid cancer. Cultured cell have grown well without interruption for over 6 years. The monolayer cultured cells appeared in epithelial in shape, showing pavement arrangement and piling up tendency without contact inhibition. The cytology revealed anaplastic and pleomorphic features. In electron-microscopy, two types of cells were observed, one with much tonofilament and desmosomes, and the other with much glycogen in cytoplasma. The chromosome studies showed aneuploidy and modal number of 74 and 75. An apparent marker chromosome was absent. The doubling time was 35-42h. and the plating efficiency was 70-88%. Heat-stable, L-phenylalanine sensitive alkaline phosphatase, or Reagan-like isoenzyme, was observed in the cultured cells. The SGK-1 cells were transplanted subcutaneously to nude mice and submucosally to immunedepressed hamster cheek pouches, and produced tumor which resembled the original tumor.