Differentiation-inducing factor for a human leukemic cell line produced by colony-stimulating factor producing human lung cancer tissue
Hatake, K.; Motoyoshi, K.; Hanamura, T.; Mukaida, N.; Kasahara, T.; Miura, Y.; Saito, M.
Experimental Hematology 15(9): 915-921
1987
ISSN/ISBN: 0301-472X PMID: 3477467 Document Number: 295446
We have found that medium conditioned by a colony-stimulating factor producing tumor derived from a granulocytosis case with lung cancer contained a factor to differentiate a human promyelocytic leukemic cell line (HL-60) to macrophage-like cells that were butyrate esterase-positive and had phagocytosing activity and membrane Fc receptors. This differentiation-inducing factor was not active for a human myeloblastic cell line (KG-1), and was separated from a colony-stimulating factor by its molecular weight and isoelectric point. The conditioned medium did not contain a detectable amount of gamma interferon when tested by bioassay as well as by radioimmunoassay. This is the first report that a human lung cancer tissue produces not only a colony-stimulating factor, but also a differentiation-inducing factor. The conditioned medium is considered to be a good source of differentiation-inducing factor.