Biologic properties of homogeneous interleukin 3. I. Demonstration of WEHI-3 growth factor activity, mast cell growth factor activity, p cell-stimulating factor activity, colony-stimulating factor activity, and histamine-producing cell-stimulating factor activity
Ihle, J.N.; Keller, J.; Oroszlan, S.; Henderson, L.E.; Copeland, T.D.; Fitch, F.; Prystowsky, M.B.; Goldwasser, E.; Schrader, J.W.; Palaszynski, E.; Dy, M.; Lebel, B.
Journal of Immunology 131(1): 282-287
1983
ISSN/ISBN: 0022-1767 PMID: 6190911 Document Number: 218491
Interleukin 3 (IL 3) was initially defined as a factor in conditioned media from concanavalin A-stimulated lymphocytes (Con A CM) that induces the enzyme 20-.alpha.-hydroxysteroid dehydrogenase (20.alpha.SDH) in cultures of nu/nu splenic lymphocytes. To determine the spectrum of additional biologic activities, IL 3 was purified to homogeneity and its properties were assessed. The protein preparation was judged to be homogeneous IL 3 by the following criteria: elution of a peak of IL 3 with a constant specific activity in the last step of purification, presence of a single protein by SDS-PAGE growth factor, mast cell growth factor and histamine-producing cell-stimulating factor. Homogeneous IL 3 had colony-stimulating factor activity, although only .apprx. 2% of the total colony-stimulating factor activity found in Con A CM was associated with IL 3. The major peak of colony-stimulating factor activity could be resolved from IL 3 by DEAE column chromatography and lacked many of the biologic activities associated with IL 3.