Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells
Müller, B.K.; Kabos, P.; Belhage, B.; Neumann, T.; Kater, S.B.
European Journal of Cell Biology 69(4): 360-367
1996
ISSN/ISBN: 0171-9335 PMID: 8741218 Document Number: 455474
Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish. to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.