Isolation of the calcium-binding domain of carp parvalbumin
Maksimov, E.E.; Mitin, I.V.
Molekuliarnaia Biologiia 11(4): 807-810
1977
ISSN/ISBN: 0026-8984 PMID: 618324 Document Number: 119061
Carp parvalbumin has two calcium-binding domains with a similar three-dimensional structure. Using the tryptic hydrolysis at the arginine residue in position 75, it was possible to split off one calcium-binding domain. All lysine residues were protected by maleic groups which were removed at the final stage. The domain (with a peptide thirty-three residues) isolated by ion-exchange chromatography and gel filtration does not have a secondary structure in a solution and is unable to bind calcium.