Toxic effect of a photo-induced tryptophan-riboflavin adduct on F9 teratocarcinoma cells and preimplantation mouse embryos
Silva, E.; Salim-Hanna, M.; Becker, M.I.; de Ioannes, A.
International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 58(4): 394-401
1988
ISSN/ISBN: 0300-9831 PMID: 3243692 Document Number: 326084
Solutions containing L-tryptophan and riboflavin exposed to visible light, under N2 atmosphere, yield a tryptophan-riboflavin adduct, able to inhibit the growth of cultured F9 teratocarcinoma cells. This same effect was found in the presence of a mixture of the tryptophan photooxidation products and the adduct, when using solutions previously irradiated with visible light in an O2 atmosphere. A cytotoxic effect was also observed with embryos incubated in the presence of a tryptophan-riboflavin adduct, in the latter case necrosis and embryo development arrest occurred.