A method for analysing sister-chromatid exchange in mouse preimplantation embryos
Elbling, L.; Colot, M.
Mutation Research 147(1-2): 23-28
1985
ISSN/ISBN: 0027-5107 PMID: 3974609 Document Number: 243190
Analysis of sister-chromatid exchange (SCE) was shown to be a sensitive and reproducible method for detecting the action of mutagens and carcinogens. A reliable technique was established which allows to perform SCE in preimplantation embryos in order to make the pre-uterine stages of development accessible to routine detection of DNA damage. Using the mouse strain and technique described, .apprx. 30-40% of mice will mate successfully after synchronization and spontaneous ovulation. From 3 pregnant females, about 30 4-8-cell embryos will be obtained, representing one experimental group providing .apprx. 50-80 2-S-phase labeled metaphases with a SCE frequency baseline below 6 exchanges.