Simultaneous estimation of sister chromatid exchange and evaluation of cell cycle delay
Chebotarev, A.N.; Golovina, M.E.; Smirnova, T.D.
Biulleten' Eksperimental'noi Biologii i Meditsiny 105(5): 589-591
1988
ISSN/ISBN: 0365-9615 PMID: 3132995 Document Number: 306988
The frequency of sister chromatid exchange and cell cycle duration were evaluated simultaneously. This approach is based on the analysis of distribution of cells with differential staining of sister chromatids after treatment of cells with 5-bromodeoxyuridine. The treatment of cells with thiotepa caused no changes in cell cycle duration, while the combination of thiotepa and hydroxyurea (HU) or cytosine-beta-D-arabinofuranoside (ARA-C) was observed to prolong cell cycle duration. Furthermore, it has been shown that caffeine, HU, ARA-C do not increase frequency of sister chromatid exchange in control cells and in cell treated with thiotepa.