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Solid phase synthesis and immunogenicity of a VP3 peptide from hepatitis a virus

Pérez, J.A.; González-Dankaart, J.F.; Reig, F.; Pintó, R.M.; Bosch, A.; Haro, I.

Biomedical Peptides Proteins and Nucleic Acids Structure Synthesis and Biological Activity 1(2): 93-100

1995

ISSN/ISBN: 1353-8616
PMID: 9346860
Document Number: 448241
The synthesis of a peptide belonging to the VP3 capsid protein of Hepatitis A virus has been accomplished by the continuous flow Fmoc-polyamide solid phase method. The use of methoxytrimethylbenzenesulphonyl (Mtr) and pentamethylchromansulphonyl (Pmc) as arginine side-chain protecting groups in the presence of tryptophan without lateral protection or protected with t-Boc is discussed. The synthetic VP3 peptide has been administered to mice in different forms: (i) free, (ii) coupled to keyhole limpet hemocyanin, (iii) encapsulated in multilamellar (MLV) liposomes, and (iv) incorporated to a tetrameric branched lysine core. The immune response induced by these preparation is reported.

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