Methods for solid phase peptide synthesis which employ a minimum of instrumentation
Edmondson, J.M.; Klebe, R.J.; Zardeneta, G.; Weintraub, S.T.; Kanda, P.
Biotechniques 6(9): 868
1988
ISSN/ISBN: 0736-6205 PMID: 3273197 Document Number: 308215
Both automated and manual methods of solid phase peptide synthesis employ three basic steps: (a) Attachment of the first amino acid to a resin, (b) peptide synthesis via successive carbodiimide couplings and (c) cleavage and deblocking of the peptide. Instead of an automated peptide synthesizer, one can manually synthesize peptides with a sintered glass funnel as the only required piece of equipment. Following solid phase synthesis, one can cleave and deblock peptides without the use of anhydrous hydrofluoric acid (HF); hence, the need for specialized equipment required for handling HF can also be eliminated. In the procedure described in this report, cleavage and deblocking is carried out with trifluoromethane sulfonic acid (TFMSA) in glass vessels without the need for high pressure Telfon fittings. Since completion of the coupling reaction can be monitored during each cycle when manual methods are employed, one can avoid repetitive couplings and, thereby economized on reagents. Since TFMSA cleavage and deblocking can be carried out in open glass vessels, one can cleave and deblock large numbers of peptides at the same time. With the methods described, one can satisfactorily prepare large quantities of peptides at minimal cost.