Expression and secretion of alpha-amylase and glucoamylase in Saccharomyces cerevisiae
Luo, J.; He, M.; Li, W.; Zhang, T.
Chinese Journal of Biotechnology 10(4): 241-248
1994
ISSN/ISBN: 1042-749X PMID: 7780020 Document Number: 434872
alpha-Amylase genes of Bacillus licheniformis and glucoamylase cDNA of Aspergillus niger were ligated to a E. coli-yeast shuttle vector. The resultant plasmid was used to transform Saccharomyces cerevisiae to construct starch-degrading yeast strain. The results of enzyme activity assay and enzyme property analysis show that alpha-amylase and glucoamylase genes have been expressed simultaneously in yeast under the control of promoters and terminators of yeast MF-alpha 1 factor and PGK genes and over 99% of enzyme activities were secreted to the medium. The engineered yeast strain hydrolyses 97% of the starch (10%) in the medium after 6 days. The recombinant plasmid exists stably in yeast.