Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae
Tuite, M.F.; Dobson, M.J.; Roberts, N.A.; King, R.M.; Burke, D.C.; Kingsman, S.M.; Kingsman, A.J.
EMBO Journal 1(5): 603-608
1982
ISSN/ISBN: 0261-4189 PMID: 6329694 Document Number: 183285
The 5' control region of the yeast phosphoglycerate kinase gene (PGK) was fused to the coding sequence of a human interferon-alpha. This PGK-interferon fusion was then introduced into yeast on a high copy number 2mu-based plasmid vector. Strains containing this plasmid produced a PGK-interferon-alpha fusion protein as 1-2% of cell protein and the expression of interferon activity was regulated by the availability of a fermentable carbon source. The system is capable of making as much as 15 mg of human interferon-alpha per litre of batch culture.