Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells
Ratovitskiĭ, E.A.; Rudenko, A.B.; Vasinova, N.A.; Kidgotko, O.V.; Nisman, B.K.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 20-24
1990
ISSN/ISBN: 0208-0613 PMID: 2199826 Document Number: 365987
The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.