Enzyme immunoassay for the determination of serotonin in stored platelet concentrates
Ganschow, I.; Benn, H.P.; Fischer, R.; Cassens, U.; Dittmer, R.; Kühnl, P.
Beitrage Zur Infusionstherapie und Transfusionsmedizin 32: 428-430
1994
ISSN/ISBN: 1023-2028 PMID: 9480138 Document Number: 429722
A new enzyme immunoassay (EIA) was applied on measurements of serotonin (5-HT) in stored platelet concentrates (PC). PC were prepared by apheresis of two separators (Cobe, Fresenius) or from buffy coats and stored for a period of 7 days at 22 degrees C and agitating. Measurements were done in pellets and suspension medium (plasma). Detection of 5-HT was specific and sensitive within the bounds of 2 and 1,000 ng/10(9) PLT.