Determination of human neutrophil elastase in bronchoalveolar lavage fluid: homogeneous immunoactivation versus heterogeneous enzyme immunoassay
Dwenger, A.; Schweitzer, G.; Pape, H.C.; Lehmann, U.
European Journal of Clinical Chemistry and Clinical Biochemistry 30(11): 785-786
1992
ISSN/ISBN: 0939-4974 PMID: 1489852 Document Number: 392724
Elastase mass concentrations of bronchoalveolar lavage fluid were determined by a homogeneous immunoactivation and a heterogeneous enzyme immunoassay. There was an excellent correlation between both assay systems (7 = 1.0376 cntdot x + 1.311; r = 0.9901; n = 43) indicating the suitability of the immunoactivation method for the determination of elastase concentrations in bronchoalveolar lavage fluid as a matrix. Furthermore, dilution of bronchoalveolar lavage fluid samples did not influence the elastase recovery of either assay system.