Quality control of stored platelet concentrates by means of flow cytometry
Böck, M.; Gawaz, M.; Heim, M.U.; Mempel, W.
Beitrage Zur Infusionstherapie und Transfusionsmedizin 32: 397-400
1994
ISSN/ISBN: 1023-2028 PMID: 9480132 Document Number: 428683
Storage of single-donor platelets is currently limited to 5 days. During this period, however, numerous morphologic and biochemical changes have been observed. The present study describes increased binding of anti-GMP 140 to stored single-donor platelet concentrates; this reveals a progressive activation process. In contrast, when stored platelets are stimulated with ADP, GMP 140, expression is reduced indicating a diminished release reaction. Additionally, the GP IIb/IIIa receptor complex and its fibrinogen-induced binding site (LIBS1) are reduced by storage time, demonstrating a diminished fibrinogen binding. Further experiments have to clarify whether these changes observed in vitro translate into a reduced hemostatic capacity after transfusion in vivo.