Increasing DNase I activity after exposure of isolated DNA to halothane
Reitz, M.; Knitza, R.; Lanz, E.
Arzneimittel-Forschung 43(2): 92-94
1993
ISSN/ISBN: 0004-4172 PMID: 8457242 Document Number: 414945
DNA was exposed to halothane (CAS 151-67-7) in a cell-free system. After exposure the DNA was used as substrate for DNase I from bovine pancreas. The DNase I activity increased after halothane exposure of the substrate depending on time and dosis. Drugs are able to influence the DNA conformation. Conformational changes in the DNA can enhance the DNase I cleavage rate. Therefore, it is possible that halothane exposure induces changes in DNA conformation demonstrable by an increased DNase I activity. The results suggest a mechanism by which halothane may contribute to chromosomal defects and disturbances of DNA metabolism in cells.