Activity of DNase associated with replication of Epstein-Barr virus in NPC-204 cells
Liu, M.Y.; Chen, J.Y.; Yang, C.S.
Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 17(3): 121-130
1984
ISSN/ISBN: 0253-2662 PMID: 6096087 Document Number: 233005
A deoxyribonuclease derived from Epstein-Barr virus (EBV)-producing lymphoblastoid cell line (NPC-204 cells) treated with IUdR was purified by DEAE cellulose, phospho-cellulose, and DNA-cellulose columns with 45% recovery. The activity of the DNase was neutralized by serum of patient with nasopharyngeal carcinoma (NPC). The DNase had a strong requirement for divalent cations and an alkaline pH optimum. Its activity was inhibited by highly ionic strength and polyamines.