A rapid and simple method for determining the DNA sequences of fragments inserted into vaccinia virus
Sheng, N.; Zhang, J.; Whitton, J.L.; McKee, T.
Biotechniques 14(5): 781-784
1993
ISSN/ISBN: 0736-6205 PMID: 8512703 Document Number: 411356
Recombinant virus vectors such as vaccinia virus, adenovirus and herpesvirus are frequently used to express a variety of foreign products. A rapid method allowing the precise identification of recombinants would be useful to confirm the nature of a newly produced recombinant and, in particular, to discriminate between recombinants bearing near-identical foreign products. Using vaccinia virus, we describe a method that in one day provides sequence analysis of the recombinant viral DNA.