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A simple sensitive method for determining staphylococcal thermonuclease in cheese

Ibrahim, G.F.

Journal of Applied Bacteriology 51(2): 307-312

1981

ISSN/ISBN: 0021-8847
PMID: 7298532
Document Number: 170854
A simple quantitative method is described for the determination of staphylococcal thermostable nuclease in cheese. The method does not require concentration or purification of the nuclease prior to determination because of the increased sensitivity of the assay (0.5 ng/ml). Assay results of cheddar cheese made from pasteurized milk inoculated with enterotoxin-A producing Staphylococcus aureus strains demonstrated the sensitivity and reliability of the method for indicating the presence of S. aureus at concentrations of .gtoreq. 1.4 .times. 106/g of cheese.

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