A rapid and reliable method to create tandem arrays of short DNA sequences
Graham, G.J.; Maio, J.J.
Biotechniques 13(5): 780-789
1992
ISSN/ISBN: 0736-6205 PMID: 1418980 Document Number: 389765
Tandemly polymerized regulatory elements, antisense RNA segments of ribozymes are potentially useful in selective gene silencing. However, existing methods of tandemly polymerizing short DNA segments are laborious. We present a procedure that can create cloned arrays of 40-70 monomer units in two steps. We have created long arrays of regulatory elements and potential ribozyme sequences. Silencing of human immunodeficiency virus (HIV-1) activation by tandem arrays of a regulatory element in human immune system cells and in other human and monkey cells is discussed.