Highly discriminating heptaplex short tandem repeat PCR system for forensic identification
Urquhart, A.; Oldroyd, N.J.; Kimpton, C.P.; Gill, P.
Biotechniques 18(1): 116-8 120-1
1995
ISSN/ISBN: 0736-6205 PMID: 7702836 Document Number: 446888
We describe a highly discriminating multiplex short tandem repeat PCR human identification system that gives a matching probability for Caucasians of European ancestry of 2.94 times 10-8 or 5.66 times 10-10 when used in combination with a previously described system. The system produces discrimination equal to or greater than four single locus probes (restriction fragment length polymorphism (RFLP) typing of variable of variable nucleotide tandem repeat (VNTRI loci). The test is robust and reproducible and works with 1-10 ng of template DNA, using fluorescent detection of PCR products from either 4 or 6 short tandem repeat loci and the X-Y homologous gene amelogenin, giving simultaneous sex diagnosis.