Effects of aging on mechanical performance of Wistar rat papillary muscles
Cicogna, A.C.; Padovani, C.R.; Nardi, S.C.; Okoshi, M.P.
Arquivos Brasileiros de Cardiologia 60(4): 215-219
1993
ISSN/ISBN: 0066-782X PMID: 8311727 Document Number: 409405
To evaluate the effects of age on mechanical performance of rat myocardium. Left ventricular papillary muscles were isolated from male Wistar rats at 1, 3, 6 and 12 months of age. Muscles were studied isometrically and isotonically, stimulated at 0.2 Hz, perfused with Krebs-Henseleit solution having an external calcium concentration of 2.52 mM, and maintained at 28 degrees C. Peak isometric developed tension was significantly higher in 1 month than 3, 6 and 12 months. Peak rate of isometric tension rise decreased substantially between 1, 3 and 12 months. Time to peak isometric developed tension showed a significant increase of both 3 and 12 months of age. Time to half relaxation increased significantly from 3 to 6 and from 3 to 12 months. Maximum rate of tension decline decreased from 3 to 6 and from 3 to 12 months. No difference in resting tension was noted among any group. Isotonically, peak shortening and time to peak shortening increased from 1 to 3 months of age. Time to half re-lengthening increased from 3 to 6 and from 3 to 12 months of age. No difference in peak shortening velocity, peak relaxation velocity and relative change in muscle length was noted among any groups. The maturation affects the mechanical performance of cardiac muscle.