Induction of chemiluminescent emission in polymorphonuclear leucocytes stimulated by Sendai virus
Olinescu, R.; Săvoiu, D.; Popescu, A.; Zuivertz, A.; Tomas, E.
Revue Roumaine de Virologie 43(3-4): 175-179
1992
ISSN/ISBN: 1018-0532 PMID: 1339206 Document Number: 389409
Sendai virus (SV) stimulates in vitro the chemiluminescent emission (CHL) of human polymorphonuclear leukocytes (LPMN). The kinetics of CHL produced by LPMN depends on the structure of stimulus, the intensity being dependent of the immunological status of the subject. CHL induced by Sendai virus is similar to that given by phorbolmyristate (PMA) as regards the induction of an early maximum. To obtain CHL significant emission, an optimum ratio between LPM concentration and Sendai virus dilution is required. The maximum effect is obtained in a large scale of dilution of Sendai virus ranging from 6,000 to 16,000 uHA.