Transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae
Takegawa, K.; Yamaguchi, S.; Kondo, A.; Iwamoto, H.; Nakoshi, M.; Kato, I.; Iwahara, S.
Biochemistry International 24(5): 849-855
1991
ISSN/ISBN: 0158-5231 PMID: 1776953 Document Number: 377281
Endo-.beta.-N-acetylglucosaminidase from Arthrobacter protophormiae has transglycosylation activity. Treatment of (Man)6(GlcNAc)2Asn with the enzyme in the presence of free N-acetylglucosamine gave a mixture of (Man)6GlcNAc and (Man)6GlcNAc.beta.1 .fwdarw. 4GlcNAc mixture. N-Acetylglucosamine at the reducing end of the latter sugar chain was found by HPLC of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 MHz 1H-NMR spectroscopy.