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Pepsin-catalyzed peptide synthesis in biphasic systems

Bemquerer, M.P.; Theobaldo, F.C.; Tominaga, M.

Biomedica Biochimica Acta 50(10-11): S94-S97

1991

ISSN/ISBN: 0232-766X
PMID: 1820069
Document Number: 374935
Pepsin catalyzed peptide synthesis in biphasic systems containing more than 95% (v/v) of organic phase was studied. Good yields were only obtained with more hydrophobic solvents, in spite of the low solubility of the substrates. The effects of the following parameters were also investigated: concentration of amino and carboxylic components, pH and buffer concentration, ratio between the aqueous and organic phases. The influence of different amino acid residues in the P2 position was investigated through the coupling between Z-Xyz-Phe-OH (where Xyz = Ala, Phe, Trp and Tyr) and Phe-OMe.

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