Glycoprotein (gp-51) antigen not associated with the bovine leukemia virus in FLK culture supernatant liquid
Surovac, V.M.; Adomaĭtene, D.I.
Ukrainskii Biokhimicheskii Zhurnal 63(4): 29-36
1991
ISSN/ISBN: 0201-8470 PMID: 1659007 Document Number: 371982
After elimination of the virus fraction non-associated with bovine leukemia virus (free) glycoprotein gp-51 antigen has been preparatively isolated from the supernatant of the FLK cell culture. For this purpose ultrafiltration through PM-30 membrane, thrice-repeated isoelectric focusing with different (pH 3.0-10.0, pH 4.0-6.0) ampholine intervals and affinity chromatography with ConA-sepharose are used. The SDS-polyacrylamide gel electrophoresis has determined it to be a homogeneous protein with molecular weight 51,000 daltons. The results of the enzyme-linked immunosorbent assay, immunoelectrophoresis and agar-gel immunodiffusion test confirm the specific activity of isolated gp-51 antigen. The gp-51 antigen may be used for identification of antibodies in the blood serum of leukemia virus of infected cattle and for monoclonal screening of antibodies.