Bovine monoclonal antibodies specific for bovine herpesvirus-1 glycoprotein gIII
Srikumaran, S.; Onisk, D.V.; Zamb, T.J.; Osorio, F.A.; Rice, D.N.
American Journal of Veterinary Research 51(4): 543-545
1990
ISSN/ISBN: 0002-9645 PMID: 2327611 Document Number: 353995
Spleen cells from a calf immunized with bovine herpesvirus-1 (BHV-1) were fused with the nonsecreting murine cell line SP2/0. Several bovine-murine hybridomas secreting bovine immunoglobulins were stabilized. Of these, 9 hybridomas secreted bovine monoclonal antibodies that specifically bound to BHV-1 in a radioimmunoassay. Two of these monoclonal antibodies reacted specifically with BHV-1 in an indirect fluorescent antibody test and immunoprecipitated a BHV-1 glycoprotein with molecular mass of 97 kilodaltons.