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Bovine papillomavirus type 1 monoclonal antibodies

Gorra, J.B.; Lancaster, W.D.; Kurman, R.J.; Jenson, A.B.

Journal of the National Cancer Institute 75(1): 121-125

1985

ISSN/ISBN: 0027-8874
PMID: 2989600
Document Number: 249252
Bovine papillomavirus type 1 (BPV-1) and type 2 (BPV-2) are the etiologic agents of fibropapillomas in cattle. Polyclonal antisera produced against BPV-1 structural antigens are cross-reactive with BPV-2. In this study BPV-1 type-specific monoclonal antibodies were produced that were not reactive with BPV-2. These monoclonal antibodies could be used for identification of BPV-1 structural antigens in acetone-fixed, frozen sections by immunofluorescence and Formalin-fixed, paraffin-embedded sections by immunoperoxidase techniques. In addition, these antibodies could be used for identification and purification of BPV-1 virions by immune electron microscopy and immunoadsorption techniques, respectively.

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