Genotypic identification of the bovine leukemia virus
Vafin, R.R.; Khazipov, N.Z.; Shaeva, A.I.; Zakirova, Z.R.; Zaĭnullin, L.I.; Tiul'kin, S.V.; Abdulina, I.R.; Alimov, A.M.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 34-40
2014
ISSN/ISBN: 0208-0613 PMID: 25845140 Document Number: 674097
Phylogenetic analysis of the sequenced segments of the provirus BLV locus env-gene and the strategy of the PCR-PDRF-genotyping consistent with phylogenetic classification of pathogenic agent and suggested in our works provided taxonomic identification of BLV isolates identified in cattle in Tatarstan (Russian Federation) as representatives of the 4th, 7th, and 8th BLV genotypes. Of 100 identified isolates, 64 represent the 4th BLV genotype, 28 representatives of BLV belong to cluster of the 7th genotype, whereas the other 8 samples of the provirus belong to the new 8th genotype of pathologic agent. The strategy VBL PCR-PDRF-genotyping suggested in our work on the basis of 5 restriction endonucleases (PvuII, SspI, HphI, HaeIII, and BstYI) provided correct genotyping identification of the viral pathogen.